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Issue 1, 2018
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Tracking iron-associated proteomes in pathogens by a fluorescence approach

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Abstract

Porphyromonas gingivalis is a key oral anaerobic bacterium involved in human periodontitis, which may affect up to 15% adults worldwide. Using the membrane permeable fluorescent probe Fe-TRACER, we identified 17 iron-associated proteins in Porphyromonas gingivalis. We demonstrated the specific binding of the probe towards iron-associated proteins using transferrin as an example and provided an X-ray structure of the fluorescent probe-bound transferrin. Our study provides a basis for the understanding of iron homeostasis in pathogens, and our approach based on the integration of fluorescence imaging with proteomics and bioinformatics can be readily extended to mine other metalloproteomes in microbials.

Graphical abstract: Tracking iron-associated proteomes in pathogens by a fluorescence approach

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Publication details

The article was received on 28 Sep 2017, accepted on 14 Dec 2017 and first published on 14 Dec 2017


Article type: Communication
DOI: 10.1039/C7MT00275K
Citation: Metallomics, 2018,10, 77-82
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    Tracking iron-associated proteomes in pathogens by a fluorescence approach

    N. Jiang, T. Cheng, M. Wang, G. C. Chan, L. Jin, H. Li and H. Sun, Metallomics, 2018, 10, 77
    DOI: 10.1039/C7MT00275K

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