Jump to main content
Jump to site search


Stable and efficient generation of poly(β-amino ester)s for RNAi delivery

Abstract

Cationic polymers are promising delivery systems for RNAi due to their ease of manipulation, scale-up conditions and their transfection efficiency. However, some properties, such as stability and targeting, remain challenging to overcome. In this report, different modifications in the poly(β-amino ester) (pBAE) structures have been explored to overcome these limitations. Recent studies have demonstrated that hydrophobicity plays a key role in controlling electrostatic interactions of plasma proteins with nanoparticles. Results show that a slight increase in the polymer hydrophobicity increases its siRNA packaging capacity, stability, and transfection efficiency. Consequently, polyplexes prepared with these hydrophobic structures are functional after incubation times longer than 48 hours in serum-containing medium. In addition, newly designed polymers were end-modified using different oligopeptides moieties in order to confer cell-specificity, as previously reported. Therefore, it can be concluded that these newly optimized pBAE polymers present a great potential as delivery vectors to specifically drive therapeutic RNA-based nucleic acids in a cell-specific manner under physiological conditions.

Back to tab navigation

Supplementary files

Publication details

The article was received on 08 Feb 2018, accepted on 17 May 2018 and first published on 17 May 2018


Article type: Paper
DOI: 10.1039/C8ME00006A
Citation: Mol. Syst. Des. Eng., 2018, Accepted Manuscript
  •   Request permissions

    Stable and efficient generation of poly(β-amino ester)s for RNAi delivery

    P. Dosta, V. Ramos-Pérez and S. Borrós, Mol. Syst. Des. Eng., 2018, Accepted Manuscript , DOI: 10.1039/C8ME00006A

Search articles by author

Spotlight

Advertisements