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Ultrahigh-throughput droplet microfluidic device for single-cell miRNA detection with isothermal amplification

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Abstract

Analysis of microRNA (miRNA), a pivotal primary regulator of fundamental cellular processes, at the single-cell level is essential to elucidate regulated gene expression precisely. Most single-cell gene sequencing methods use the polymerase chain reaction (PCR) to increase the concentration of the target gene for detection, thus requiring a barcoding process for cell identification and creating a challenge for real-time, large-scale screening of sequences in cells to rapidly profile physiological samples. In this study, a rapid, PCR-free, single-cell miRNA assay is developed from a continuous-flow microfluidic process employing a DNA hybridization chain reaction to amplify the target miRNA signal. Individual cells are encapsulated with DNA amplifiers in water-in-oil droplets and then lysed. The released target miRNA interacts with the DNA amplifiers to trigger hybridization reactions, producing fluorescence signals. Afterward, the target sequences are recycled to trigger a cyclic cascade reaction and significantly amplify the fluorescence signals without using PCR thermal cycling. Multiple DNA amplifiers with distinct fluorescence signals can be encapsulated simultaneously in a droplet to measure multiple miRNAs from a single cell simultaneously. Moreover, this process converts the lab bench PCR assay to a real-time droplet assay with the post-reaction fluorescence signal as a readout to allow flow cytometry-like continuous-flow measurement of sequences in a single cell with an ultrahigh throughput (300–500 cells per minute) for rapid biomedical identification.

Graphical abstract: Ultrahigh-throughput droplet microfluidic device for single-cell miRNA detection with isothermal amplification

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Publication details

The article was received on 14 Apr 2018, accepted on 30 May 2018 and first published on 30 May 2018


Article type: Paper
DOI: 10.1039/C8LC00390D
Citation: Lab Chip, 2018, Advance Article
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    Ultrahigh-throughput droplet microfluidic device for single-cell miRNA detection with isothermal amplification

    S. Guo, W. N. Lin, Y. Hu, G. Sun, D. Phan and C. Chen, Lab Chip, 2018, Advance Article , DOI: 10.1039/C8LC00390D

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