Issue 14, 2018

Real-time observation of leukocyte–endothelium interactions in tissue-engineered blood vessel

Abstract

Human cell-based 3D tissue constructs play an increasing role in disease modeling and drug screening. Inflammation, atherosclerosis, and many autoimmune disorders involve the interactions between immune cells and blood vessels. However, it has been difficult to image and model these interactions under realistic conditions. In this study, we fabricated a perfusion and imaging chamber to allow the real-time visualization of leukocyte perfusion, adhesion, and migration inside a tissue-engineered blood vessel (TEBV). We monitored the elevated monocyte adhesion to the TEBV wall and transendothelial migration (TEM) as the TEBV endothelium was activated by the inflammatory cytokine TNF-α. We demonstrated that treatment with anti-TNF-α or an NF-kB signaling pathway inhibitor would attenuate the endothelium activation and reduce the number of leukocyte adhesion (>74%) and TEM events (>87%) close to the control. As the first demonstration of real-time imaging of dynamic cellular events within a TEBV, this work paves the way for drug screening and disease modeling in TEBV-associated microphysiological systems.

Graphical abstract: Real-time observation of leukocyte–endothelium interactions in tissue-engineered blood vessel

Supplementary files

Article information

Article type
Paper
Submitted
23 Feb 2018
Accepted
26 May 2018
First published
01 Jun 2018

Lab Chip, 2018,18, 2047-2054

Real-time observation of leukocyte–endothelium interactions in tissue-engineered blood vessel

Z. Chen, M. Tang, D. Huang, W. Jiang, M. Li, H. Ji, J. Park, B. Xu, L. J. Atchison, G. A. Truskey and K. W. Leong, Lab Chip, 2018, 18, 2047 DOI: 10.1039/C8LC00202A

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements