Jump to main content
Jump to site search

“Dip-and-read” paper-based analytical devices using distance-based detection with colour screening


An improved microfluidic paper-based analytical device (PAD) using colour screening to enhance device performance is described. Current detection methods for PADs relying on the distance-based signaling motif can be slow due to the assay time being limited by capillary flow rates that wick fluid through the detection zone. For traditional distance-based detection motifs, analysis can take up to 45 min for a channel length of 5 cm. By using a colour screening method, quantitation using distance-based PAD can be achieved in minutes using a “dip-and-read” approach. A colorimetric indicator line deposited onto a paper substrate using inkjet-printing undergoes a concentration-dependent colorimetric response for a given analyte. This colour intensity-based response has been converted to a distance-based signal by overlaying a colour filter with a continuous colour intensity gradient matching the colour of the developed indicator line. As a proof-of-concept, Ni quantification in welding fume was performed as a model assay. The results of multiple independent user testing gave the mean absolute percentage error and average relative standard deviations of 10.5% and 11.2% respectively, which were an improvement upon analysis based on simple visual colour comparison with a read guide (12.2%, 14.9%). In addition to the analytical performance comparison, an interference study and a shelf life investigation were performed to further demonstrate practical utility. The developed system demonstrates an alternative detection approach for distance-based PADs enabling fast (~ 10 min), quantitative, and straightforward assays.

Back to tab navigation

Supplementary files

Publication details

The article was received on 09 Feb 2018, accepted on 29 Mar 2018 and first published on 05 Apr 2018

Article type: Paper
DOI: 10.1039/C8LC00168E
Citation: Lab Chip, 2018, Accepted Manuscript
  •   Request permissions

    “Dip-and-read” paper-based analytical devices using distance-based detection with colour screening

    K. Yamada, D. Citterio and C. Henry, Lab Chip, 2018, Accepted Manuscript , DOI: 10.1039/C8LC00168E

Search articles by author