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Issue 8, 2018
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Fluorescent analysis of bioactive molecules in single cells based on microfluidic chips

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Abstract

Single-cell analysis of bioactive molecules is an essential strategy for a better understanding of cell biology, exploring cell heterogeneity, and improvement of the ability to detect early diseases. In single-cell analysis, highly efficient single-cell manipulation techniques and high-sensitive detection schemes are in urgent need. The rapid development of fluorescent analysis techniques combined with microfluidic chips have offered a widely applicable solution. Thus, in this review, we mainly focus on the application of fluorescence methods in components analysis on microchips at a single-cell level. By targeting different types of biological molecules in cells such as nucleic acids, proteins, and active small molecules, we specially introduce and comment on their corresponding fluorescent probes, fluorescence labelling and sensing strategies, and different fluorescence detection instruments used in single-cell analysis on a microfluidic chip. We hope that through this review, readers will have a better understanding of single-cell fluorescence analysis, especially for single-cell component fluorescence analysis based on microfluidic chips.

Graphical abstract: Fluorescent analysis of bioactive molecules in single cells based on microfluidic chips

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Publication details

The article was received on 13 Dec 2017, accepted on 27 Feb 2018 and first published on 07 Mar 2018


Article type: Tutorial Review
DOI: 10.1039/C7LC01333G
Citation: Lab Chip, 2018,18, 1151-1173
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    Fluorescent analysis of bioactive molecules in single cells based on microfluidic chips

    Y. Fan, D. Dong, Q. Li, H. Si, H. Pei, L. Li and B. Tang, Lab Chip, 2018, 18, 1151
    DOI: 10.1039/C7LC01333G

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