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Injection molded lab-on-a-disc platform for screening of genetically modified E. coli using liquid–liquid extraction and surface enhanced Raman scattering

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Abstract

We present the development of an automated centrifugal microfluidic platform with integrated sample pre-treatment (filtration and liquid–liquid extraction) and detection (SERS-based sensing). The platform consists of eight calibration and four assay modules, fabricated with polypropylene using injection molding and bonded with ultrasonic welding. The platform was used for detection of a secondary bacterial metabolite (p-coumaric acid) from bacterial supernatant. The obtained extraction efficiency was comparable to values obtained in batch experiments and the SERS-based sensing showed a good correlation with HPLC analysis.

Graphical abstract: Injection molded lab-on-a-disc platform for screening of genetically modified E. coli using liquid–liquid extraction and surface enhanced Raman scattering

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Publication details

The article was received on 14 Nov 2017, accepted on 09 Feb 2018 and first published on 09 Feb 2018


Article type: Paper
DOI: 10.1039/C7LC01217A
Citation: Lab Chip, 2018, Advance Article
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    Injection molded lab-on-a-disc platform for screening of genetically modified E. coli using liquid–liquid extraction and surface enhanced Raman scattering

    L. Morelli, L. Serioli, F. A. Centorbi, C. B. Jendresen, M. Matteucci, O. Ilchenko, D. Demarchi, A. T. Nielsen, K. Zór and A. Boisen, Lab Chip, 2018, Advance Article , DOI: 10.1039/C7LC01217A

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