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Combination of Micro X-Ray Fluorescence Spectroscopy and Time-of-Flight Secondary Ion Mass Spectrometry Imaging for the Marker-free Detection of NM212 CeO2 Nanoparticles in Tissue Sections

Abstract

The description of local effects of nanoparticles (NP) in lung tissue is a current goal in nanotoxicological studies. Three-dimensional Time-of-Flight-Secondary Ion Mass Spectrometry (ToF-SIMS 3D) is a promising label-free method to analyse tissue sections for nanoparticle distribution and to simultaneously detect organic and inorganic molecule species at high spatial resolution. As sample regions accessible to ToF-SIMS 3D imaging are limited to several hundred square micrometres, the proper selection of nanoparticle containing regions is mandatory. Micro X-Ray Fluorescence Spectrometry (µ-XRF) provides a non-destructive, fast elemental analysis of large tissue sections with lateral resolution in the micrometer range. The latter technique was, therefore, used to screen samples as well as to validate elemental distribution images from ToF-SIMS 3D analysis. Specifically, cryo-sections from lungs inhomogeneously laden with CeO2 nanoparticles (10-200 nm) were subjected to µ-XRF and reflected light microscopy to pre-select lung tissue areas containing CeO2 NP. The ToF-SIMS imaging analyses (lateral resolutions of 670 nm) of these areas exceed the lateral resolution of µ-XRF imaging by a factor of 42 and reveal the lateral distribution of the CeO2 NP within the tissue structure. In addition, simultaneously acquired, further secondary ions at mass resolutions of R = 4000 enlightened the sample composition. In summary, the investigation shows that µ-XRF is highly suitable to identify relevant tissue regions for subsequent high resolution ToF-SIMS 3D microanalysis of nanoparticle-laden tissue sections.

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Publication details

The article was received on 28 Sep 2017, accepted on 30 Jan 2018 and first published on 30 Jan 2018


Article type: Paper
DOI: 10.1039/C7JA00325K
Citation: J. Anal. At. Spectrom., 2018, Accepted Manuscript
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    Combination of Micro X-Ray Fluorescence Spectroscopy and Time-of-Flight Secondary Ion Mass Spectrometry Imaging for the Marker-free Detection of NM212 CeO2 Nanoparticles in Tissue Sections

    L. Veith, D. Dietrich, A. Vennemann, D. Breitenstein, C. Engelhard, U. Karst, M. Sperling, M. Wiemann and B. Hagenhoff, J. Anal. At. Spectrom., 2018, Accepted Manuscript , DOI: 10.1039/C7JA00325K

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