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Electroformation of Phospholipid Giant Unilamellar Vesicles in Physiological Phosphate Buffer

Abstract

Phospholipid Giant Unilamellar Vesicles (GUVs) are usually prepared by electroformation in water, that is in a low-conductivity solution. We developed a protocol allowing their electroformation in the most common physiological buffer, the phosphate-buffered saline (PBS, 330 mOsm, pH 7.4). This was achieved based on a specific sequence of increasing electrical fields and for the two usual electrode types for electroformation, namely Indium Tin Oxide-coated glass slides and Pt electrodes. These GUVs are stable over time (hour time-scale), they can be isolated or micro-injected. The membrane composition was modified by adding cholesterol in order to adjust its mechanical properties. The optimal proportion of cholesterol vs. total phospholipid concentration was 20 mol.% ratio, which increases membrane rigidity and facilitates vesicle microinjection.

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Publication details

The article was received on 26 Apr 2018, accepted on 13 Jun 2018 and first published on 13 Jun 2018


Article type: Technical Innovation
DOI: 10.1039/C8IB00074C
Citation: Integr. Biol., 2018, Accepted Manuscript
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    Electroformation of Phospholipid Giant Unilamellar Vesicles in Physiological Phosphate Buffer

    P. Lefrançois, B. Goudeau and S. ARBAULT, Integr. Biol., 2018, Accepted Manuscript , DOI: 10.1039/C8IB00074C

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