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Chitosan nanoparticles functionality as redox active drugs in cytotoxicity radical scavenging and cellular behaviour

Abstract

Targeting oxidative stress response has recently emerged as a promising strategy for the development of therapeutic drugs in a broad spectrum of diseases. Supporting this we have reported that chitosan nanoparticles with controlled size synthesis had selective cytotoxicity in leukemia cells underlying the mechanism related to reactive oxygen species (ROS) generation. Here in, we found that cellular uptake of chitosan nanoparticles was enhanced in a time dependent manner and inhibited cellular proliferation of leukemia cells in a dose dependent manner with elevation of reactive oxygen species (ROS) showing stronger effect on apoptosis, associated with upregulation of caspase activity and depletion of reduced glutathione. Propidium iodide and calcein staining demonstrated the central role of chitosan nanoparticles in triggering elevated ROS, inducing cell death, detecting intracellular oxidative activity. The enhanced free radical scavenging activity of chitosan nanoparticles further iterates its antioxidant activity. An in vitro quantitative phase imaging studies at the single cell level further demonstrated the inhibition of cellular proliferation with significant changes in cellular behavior and supported our hypothesis. Hemocompatibilty tests demonstrated that chitosan nanoparticles could be used safely for in vivo applications. Our findings suggest that chitosan nanoparticles may be a promising redox active candidate against therapeutic applications

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Supplementary files

Publication details

The article was accepted on 06 Apr 2018 and first published on 17 Apr 2018


Article type: Paper
DOI: 10.1039/C8IB00038G
Citation: Integr. Biol., 2018, Accepted Manuscript
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    Chitosan nanoparticles functionality as redox active drugs in cytotoxicity radical scavenging and cellular behaviour

    S. Sarangapani, A. patil, Y. K. Ngeow, R. Mohan, A. K. Asundi and M. Lang, Integr. Biol., 2018, Accepted Manuscript , DOI: 10.1039/C8IB00038G

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