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Detection of microalgae superoxide dismutase (SOD) using GNRs-based resonance light scattering system.

Abstract

A novel and label-free, gold nanorods (GNRs)-based resonance light scattering system assay has been developed for the detection of microalgae superoxide dismutase (SOD). The method relied on the basis of that interactions between microalgae SOD and GNRs could emit strong fluorescence signals. Prepared GNRs have been well dispersed in the solution and extracted from Microcystis aeruginosa intracellular SOD; SOD had been then absorbed on the surface of GNRs. The results demonstrated that the intensity of fluorescence signals increased at 595 nm with the raise of SOD concentrations, the optimum pH value has been concluded at 7.8, the optimum concentration of inorganic salt ions Na+ at 0.2mol·L-1, and the stable time of the reaction system was 50 min. This method offers the advantages of higher sensitivity and selectivity in microalgae protein detection and illustrating great potential for biology diagnosis.

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Publication details

The article was received on 01 Dec 2017, accepted on 06 Feb 2018 and first published on 06 Feb 2018


Article type: Paper
DOI: 10.1039/C7IB00206H
Citation: Integr. Biol., 2018, Accepted Manuscript
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    Detection of microalgae superoxide dismutase (SOD) using GNRs-based resonance light scattering system.

    S. Liu and Y. Tan, Integr. Biol., 2018, Accepted Manuscript , DOI: 10.1039/C7IB00206H

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