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Issue 3, 2018
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Detection of microalgae superoxide dismutase (SOD) using a GNRs-based resonance light scattering system

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Abstract

A novel and label-free, gold nanorods (GNRs)-based resonance light scattering system assay has been developed for the detection of microalgae superoxide dismutase (SOD). The method relies on the fact that interactions between microalgae SOD and GNRs can emit strong fluorescence signals. The prepared GNRs were well dispersed in the solution and intracellular SOD was extracted from Microcystis aeruginosa; the SOD was then absorbed on the surface of the GNRs. The results demonstrated that the intensity of the fluorescence signals increased at 595 nm with an increase in the SOD concentration, the optimum pH value was concluded to be 7.8, the optimum concentration of inorganic salt Na+ ions was 0.2 mol Lāˆ’1, and the reaction system was stable for 50 min. This method offers the advantages of higher sensitivity and selectivity in microalgae protein detection and exhibits great potential for biological diagnosis.

Graphical abstract: Detection of microalgae superoxide dismutase (SOD) using a GNRs-based resonance light scattering system

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Publication details

The article was received on 01 Dec 2017, accepted on 06 Feb 2018 and first published on 06 Feb 2018


Article type: Paper
DOI: 10.1039/C7IB00206H
Citation: Integr. Biol., 2018,10, 159-165
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    Detection of microalgae superoxide dismutase (SOD) using a GNRs-based resonance light scattering system

    S. Liu and Y. Tan, Integr. Biol., 2018, 10, 159
    DOI: 10.1039/C7IB00206H

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