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Molecular tools for selective recovery and detection of lignin-derived molecules

Abstract

Pulp and paper industry together with lignocellulosic biofuel production provide plentiful streams of lignin and lignin-derived molecules (LDMs) that currently remain underutilized. The heterogeneity and complexity of lignin along with the lack of convenient tools significantly hamper its utilization. Selective separation of these LDMs from streams by specific tools would allow the recovery of the aromatic compounds, as well as facilitate biological processes aiming at lignin valorization. To this end, here we report the isolation and characterization of single-chain variable fragment (scFv) antibodies against ferulate, coumarate, and caffeate, which are molecular representatives of LDMs. Binders for the target LDMs were enriched by interrogating a synthetic scFv library with phage display technique. As a result, scFv binders specific against each of the target molecule were obtained with affinities in micromolar range. The selectivity of scFvs towards specific LDMs was proven by recovering caffeate from simulated LDM solution, Kraft lignin, and rice straw hydrolysate samples. Further proof of concept studies with model compounds demonstrated the applicability of the antibody-based binders as a detection tool for monitoring microbial LDM conversion. Overall, this study demonstrates the potential of scFv binders as a specific toolset for lignin compound recovery and analytics.

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Publication details

The article was received on 12 Feb 2018, accepted on 08 May 2018 and first published on 08 May 2018


Article type: Paper
DOI: 10.1039/C8GC00490K
Citation: Green Chem., 2018, Accepted Manuscript
  • Open access: Creative Commons BY license
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    Molecular tools for selective recovery and detection of lignin-derived molecules

    M. M. Salmela, H. Sanmark, E. Efimova, A. Efimov, V. P. Hytönen, U. Lamminmäki, S. Santala and V. Santala, Green Chem., 2018, Accepted Manuscript , DOI: 10.1039/C8GC00490K

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