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Anti-inflammatory molecular mechanism of action of α-Mangostin, the major xanthone from the pericarp of Garcinia mangostana; an in silico, in vitro and in vivo approach

Abstract

α-mangostin (αMN) is a xanthone present in the pericarp of Garcinia mangostana Linn. which is mentioned in the Ayurveda and widely used functional food supplement. However, its anti-inflammatory mechanism is not well studied. Hence we used in silico, in vitro and in vivo models to provide information of mechanism on how αMN could prevent inflammation. Molecular docking was used to find out the binding energy of αMN with NFkB and COX proteins. Secondly, LPS induced RAW 264.7 cells was used to measure the production of cytokines, prevention of translocation of NFkB and inhibition of COX-1 and -2 enzymes. Finally, carrageenan-induced peritonitis was used in vivo to check the cytokine release, leukocyte migration and vascular permeability. The in silico modelling had showed that αMN has lowest binding energy with COX-2 and NFkB proteins. αMN has been found to be inhibit the production of PGE2, nitric oxide, iNOS protein expression. TNF-α and IL-6 cytokines were inhibited significantly (p<0.05) at 8 and 14 µg/ml concentration. αMN at higher doses inhibit the translocation of NFkB together with suppressing the COX-2 enzymes, but not COX-1. αMN inhibited the total leukocyte migration; predominantly, neutrophils in vivo. The level of TNFα and IL-1β was significantly (p<0.05) reduced in the peritoneal fluids as measure by ELIZA analysis. Taken together, these results demonstrate that αMN acts well as an anti-inflammatory agent via inhibiting hallmark mechanisms of inflammation. It can be considered as potential alternative leads compound. In addition, the current results support the traditional use of this fruit pericarp as a functional food.

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Publication details

The article was received on 08 Mar 2018, accepted on 30 May 2018 and first published on 01 Jun 2018


Article type: Paper
DOI: 10.1039/C8FO00439K
Citation: Food Funct., 2018, Accepted Manuscript
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    Anti-inflammatory molecular mechanism of action of α-Mangostin, the major xanthone from the pericarp of Garcinia mangostana; an in silico, in vitro and in vivo approach

    S. Mohan, S. syam, S. I. Abdelwahab and N. Thangavel, Food Funct., 2018, Accepted Manuscript , DOI: 10.1039/C8FO00439K

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