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Characterization of active antiplatelet chemical compositions of edible Citrus limon through ultra-performance liquid chromatography single quadrupole mass spectrometry-based chemometrics

Abstract

Citrus limon L. (lemon, family: Rutaceae), is the third most popular edible fruits among Citrus species, our previous work has showed the significant antiplatelet activity of lemon extracts. The aim of the present study was to identify features (retention time, m/z) associated with antiplatelet activity of lemon with a strategy to correlate platelet aggregation assay with ultra-performance liquid chromatography single quadrupole mass spectrometry-based chemometrics analysis. Primary bioactivity guided test results revealed the butanol (BA) and ethyl acetate (EA) liquid-liquid extraction sections from ethanol extract of lemon had significant inhibitory effects on platelet aggregation. Further separating BA and EA combined section with silica column, four different active fractions were obtained and their LC-MS data were collected. After being modeled by two multivariate statistical techniques, principal component analysis and orthogonal partial least squares discriminate analysis, total seven markers were predicted and identified, which were tentatively identified as priority markers for the bioactivity related in lemon. Among them, four available compounds including oxypeucedanin hydrate, citric acid, diosmin and limetin, had also been confirmed for their antiplatelet activities below 300 μM. Moreover, the specific mechanism of limetin for interacting with TP β receptor for a thromboxane A2 and then affecting the PI3K/Rap-1b signaling pathway through the βγ subunit of GPCR (i) on platelet was studied by differential proteomic analysis, to illustrate the validity and persistence of these markers for application in lemon fruit platforms.

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Publication details

The article was received on 28 Feb 2018, accepted on 04 Apr 2018 and first published on 04 Apr 2018


Article type: Paper
DOI: 10.1039/C8FO00403J
Citation: Food Funct., 2018, Accepted Manuscript
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    Characterization of active antiplatelet chemical compositions of edible Citrus limon through ultra-performance liquid chromatography single quadrupole mass spectrometry-based chemometrics

    Q. Zhang, C. Tan, L. Cai, F. Xia, D. Gao, F. Yang, H. Chen and Z. Xia, Food Funct., 2018, Accepted Manuscript , DOI: 10.1039/C8FO00403J

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