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Issue 5, 2018
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Characterization of active antiplatelet chemical compositions of edible Citrus limon through ultra-performance liquid chromatography single quadrupole mass spectrometry-based chemometrics

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Abstract

Citrus limon L. (lemon, family: Rutaceae) is the third most popular edible fruit among the Citrus species. Our previous study has shown the significant antiplatelet activity of lemon extracts. The aim of the present study is to identify the features (retention time, m/z) associated with the antiplatelet activity of lemons by correlating a platelet aggregation assay with ultra-performance liquid chromatography single quadrupole mass spectrometry-based chemometrics analysis. The primary bioactivity-guided test results revealed that the butanol (BA) and ethyl acetate (EA) liquid–liquid extraction sections of the ethanol extract of lemons had significant inhibitory effects on platelet aggregation. Upon further separating the combined BA and EA sections with a silica column, four different active fractions were obtained, and their LC-MS data were collected. After modeling by two multivariate statistical techniques, namely, principal component analysis and orthogonal partial least squares discriminate analysis seven markers were predicted, identified, and tentatively classified as priority markers of bioactivity in lemons. Among them, the antiplatelet activity of four marker compounds, namely, oxypeucedanin hydrate, citric acid, diosmin, and limetin at concentrations lower than 300 μM was confirmed. Moreover, the specific mechanism of limetin interaction with the TP β receptor of thromboxane A2 and the effect of limetin on the PI3 K/Rap-1b signaling pathway through the βγ subunit of GPCR (i) in platelet aggregation were studied by differential proteomic analysis to illustrate the validity and persistence of these markers for application in lemon fruit platforms.

Graphical abstract: Characterization of active antiplatelet chemical compositions of edible Citrus limon through ultra-performance liquid chromatography single quadrupole mass spectrometry-based chemometrics

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Publication details

The article was received on 28 Feb 2018, accepted on 04 Apr 2018 and first published on 04 Apr 2018


Article type: Paper
DOI: 10.1039/C8FO00403J
Citation: Food Funct., 2018,9, 2762-2773
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    Characterization of active antiplatelet chemical compositions of edible Citrus limon through ultra-performance liquid chromatography single quadrupole mass spectrometry-based chemometrics

    Q. Zhang, C. Tan, L. Cai, F. Xia, D. Gao, F. Yang, H. Chen and Z. Xia, Food Funct., 2018, 9, 2762
    DOI: 10.1039/C8FO00403J

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