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Amaranth peptides decreased the activity and expression of cellular tissue factor on LPS activated THP-1 human monocytes

Abstract

The effect of amaranth peptides on the activity and expression of tissue factor (TF) on THP-1 activated cells was evaluated in vitro. An active anticoagulant peptide fraction (AF) was found to inhibit TF expression (IC50 = 0.39 mg/mL) and activity. Immunocytochemical fluorescence confocal microscopy analysis showed that treated monocytes decreased TF membrane translocation by 49.0% and increased two-fold in nuclei compared to a positive control, indicating a decrease of active TF to initiate the coagulation cascade. Moreover, a cytokine array suggested that AF mechanism of action implied the inhibition of NF-κB pathway. Expression of MIP-3α, interleukin-1β, interleukin-1α, TARC, pentaxin 3, and PDGF-AA cytokokines was highly suppressed by AF peptides, producing reductions of 78.8%, 61.8%, 54.1%, 42.6%, 37.9% and 37.8%, respectively compared to a positive control. The results suggest a potential mechanism for the antithrombotic and anti-inflammatory effect of AF, by showing that amaranth peptides play a negative feedback regulatory role over the NF-κB pathway. In this research, we link for the first time the immunomodulatory activity of amaranth peptides with the inhibition of TF expression and therefore its antithrombotic potential.

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Publication details

The article was received on 18 Feb 2018, accepted on 29 May 2018 and first published on 30 May 2018


Article type: Paper
DOI: 10.1039/C8FO00323H
Citation: Food Funct., 2018, Accepted Manuscript
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    Amaranth peptides decreased the activity and expression of cellular tissue factor on LPS activated THP-1 human monocytes

    A. C. Sabbione, D. Luna-Vital, A. Scilingo, M. C. Añón and E. GONZALEZ DE MEJIA, Food Funct., 2018, Accepted Manuscript , DOI: 10.1039/C8FO00323H

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