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Blood-pressure lowering efficacy of winged bean seed hydrolysate in spontaneously hypertensive rats, peptide characterization and toxicity study in Sprague-Dawley rats

Abstract

Winged bean seed (WBS) is an underutilized tropical crop. Current study evaluates its potential to reduce blood pressure (BP) in spontaneously hypertensive rats and finds that it reduces BP significantly, in a dose-dependent manner. Five peptides with the sequences: RGVFPCLK, TQLDLPTQ, EPALVP, MRSVVT and DMKP, have been characterized in terms of their stability against ACE via in vitro and in silico modelling. All peptides exhibited IC50 values between 0.019 - 6.885 mM and various inhibitory modes, including substrate, prodrug and true inhibitor modes. The toxicity status of non-Current Good Manufacturing Practice (non-CGMP) peptide is evaluated and results show that such peptides are toxic, thus are not suitable to be tested in animals, particularly during long term study. In short, WBS hydrolysate demonstrated in vitro ACE inhibitory property and in vivo blood pressure lowering efficacy in rat models, fostering its potential as a functional food ingredient. Non-CGMP grade peptide is toxic and unfit for testing in animal models.

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Publication details

The article was received on 10 Nov 2017, accepted on 26 Jan 2018 and first published on 31 Jan 2018


Article type: Paper
DOI: 10.1039/C7FO01769C
Citation: Food Funct., 2018, Accepted Manuscript
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    Blood-pressure lowering efficacy of winged bean seed hydrolysate in spontaneously hypertensive rats, peptide characterization and toxicity study in Sprague-Dawley rats

    S. Y. Chay, A. Salleh, N. F. Sulaiman, N. Zainal Abidin, M. A. Hanafi, M. Zarei and N. Saari, Food Funct., 2018, Accepted Manuscript , DOI: 10.1039/C7FO01769C

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