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The neuroprotective and antioxidant profiles of selenium-containing polysaccharides from the fruits of Rosa laevigata

Abstract

Rosa laevigata fruit is a functional foodstuff for a long time, at present, increasing attention has been given to polysaccharides from R. laevigata fruit due to their numerous medicinal and nutritional properties. In this study, a rapid and effective approach of extraction and separation for polysaccharides from the title fruit was developed by microwave-assisted aqueous two-phase extraction (MA-ATPE) using PEG/ammonium sulfate system. After analysis of the response surface methodology (RSM) data based on Box-Behnken Design (BBD), a proposed model was obtained to predict an optimum value of 258.99 mg/g which is in good conformity with experiment value (258.59 mg/g). Two selenium (Se)-containing polysaccharides, Se-RLFP-1 and Se-RLFP-2, were isolated from R. laevigata fruits. Their chemical structures were elucidated by acid hydrolysis, weight-average molecular mass and Se content analysis, along with UV, FT-IR, 1H and 13C NMR spectroscopy. As a result, Se-RLFP-1 was mainly composed of mannose, glucose, galactose and xylose in a molar ratio of 1.4:7.9:1.0:1.5, while Se-RLFP-2 was composed of mannose, rhamnose, glucose, galactose and xylose (12.6:1.0:38.3:5.6:19.6). Furthermore, the antioxidant properties of them were investigated on the basis of FRAP, ABTS and DPPH radical scavenging assays. The results expressed that two polysaccharides had a noticeable effect on radical scavenging of ABTS and DPPH, especially at high concentrations. In addition, the neuroprotective effect of Se-RLFP-1 and Se-RLFP-2 against oxidative stress induced by H2O2 in SH-SY5Y neuroblastoma cells was also investigated. Particularly, the Se-RLFP-1 exhibited obvious neuroprotective activity at the concentration of 100 μg/mL.

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Publication details

The article was received on 02 Nov 2017, accepted on 15 Feb 2018 and first published on 15 Feb 2018


Article type: Paper
DOI: 10.1039/C7FO01725A
Citation: Food Funct., 2018, Accepted Manuscript
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    The neuroprotective and antioxidant profiles of selenium-containing polysaccharides from the fruits of Rosa laevigata

    X. liu, Y. Gao, D. Li, C. Liu, M. Jin, J. Bian, M. Lv, Y. Sun, L. Zhang and P. Gao, Food Funct., 2018, Accepted Manuscript , DOI: 10.1039/C7FO01725A

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