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Issue 3, 2018
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The neuroprotective and antioxidant profiles of selenium-containing polysaccharides from the fruit of Rosa laevigata

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Abstract

Rosa laevigata fruit has been known as a functional foodstuff for a long time. Recently, increasing attention has been given to polysaccharides from R. laevigata fruit due to their numerous medicinal and nutritional properties. In this study, a rapid and effective approach for the extraction and separation of polysaccharides from the title fruit was developed using microwave-assisted aqueous two-phase extraction (MA-ATPE) with a PEG/ammonium sulfate system. After analysis of the response surface methodology (RSM) data based on a Box–Behnken design (BBD), a model was proposed and was found to predict an optimum yield value of 258.99 mg g−1 which is in good agreement with the experimental value (258.59 mg g−1). Two selenium (Se)-containing polysaccharides, Se-RLFP-1 and Se-RLFP-2, were isolated from R. laevigata fruit. Their chemical structures were elucidated by acid hydrolysis, weight-average molecular mass and Se-content analysis, along with UV, FT-IR, 1H and 13C NMR spectroscopy. As a result, Se-RLFP-1 was found to be mainly composed of mannose, glucose, galactose and xylose in a molar ratio of 1.4 : 7.9 : 1.0 : 1.5, while Se-RLFP-2 was composed of mannose, rhamnose, glucose, galactose and xylose (12.6 : 1.0 : 38.3 : 5.6 : 19.6). Furthermore, the antioxidant properties of the polysaccharides were investigated on the basis of FRAP, ABTS and DPPH radical scavenging assays. The results showed that the two polysaccharides had a noticeable effect on the radical scavenging of ABTS and DPPH, especially at high concentrations. In addition, the neuroprotective effect of Se-RLFP-1 and Se-RLFP-2 against oxidative stress induced by H2O2 in SH-SY5Y neuroblastoma cells was also investigated. In particular, Se-RLFP-1 exhibited obvious neuroprotective activity at a concentration of 100 μg mL−1.

Graphical abstract: The neuroprotective and antioxidant profiles of selenium-containing polysaccharides from the fruit of Rosa laevigata

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Publication details

The article was received on 02 Nov 2017, accepted on 15 Feb 2018 and first published on 15 Feb 2018


Article type: Paper
DOI: 10.1039/C7FO01725A
Citation: Food Funct., 2018,9, 1800-1808
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    The neuroprotective and antioxidant profiles of selenium-containing polysaccharides from the fruit of Rosa laevigata

    X. Liu, Y. Gao, D. Li, C. Liu, M. Jin, J. Bian, M. Lv, Y. Sun, L. Zhang and P. Gao, Food Funct., 2018, 9, 1800
    DOI: 10.1039/C7FO01725A

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