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Effects of a galacto-oligosaccharide-rich diet on fecal microbiota and metabolite profiles in mice

Abstract

Galacto-oligosaccharides (GOS) are prebiotics that positively affect the host's gut microbiota, which is important for the health of the host. Most previous studies focused on specific flora components (e.g. Bifidobacterium and Lactobacillus); very few have investigated the relationship between flora and metabolites. Here, we used 16S rRNA analysis and metabolomics to analyze the effect of GOS on microbiota and metabolites. Results show that the abundance of Ruminococcaceae and Oscillibacter decreased significantly in GOS-fed mice. Twenty-one metabolites, including oleic acid, arachidic acid, and behenic acid, decreased significantly in GOS-fed mice. Fatty acid synthesis and blood triglyceride content significantly decreased in GOS-fed mice compared with that in the control, suggesting that GOS may improve lipid metabolism in mice. Additionally, after three weeks of a GOS-rich diet, the mouse microbiota was significantly enriched in Alloprevotella, Bacteroides, and Parasutterella. The blood glucose level of the GOS-fed group was significantly higher than that of the control group, whereas the abundance of Coprococcus and Odoribacter (butyrate-producing bacteria) was significantly decreased. The metabolism of butyrate, known to reduce plasma glucose levels, was significantly downregulated in GOS-fed mice, an effect potentially detrimental to glucose metabolism of the host. This dual-omics analysis provided important information on the changes in host-microbe- metabolite interactions resulting from GOS supplementation. Our results provide evidence that GOS may improve lipid metabolism, and that long-term GOS supplementation had a detrimental effect on the host’s glucose metabolism, which could be important for optimizing methods of prebiotic supplementation and developing approaches to prevent disease using prebiotic interventions.

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Supplementary files

Publication details

The article was received on 01 Nov 2017, accepted on 27 Jan 2018 and first published on 31 Jan 2018


Article type: Paper
DOI: 10.1039/C7FO01720K
Citation: Food Funct., 2018, Accepted Manuscript
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    Effects of a galacto-oligosaccharide-rich diet on fecal microbiota and metabolite profiles in mice

    W. Cheng, J. Lu, W. Lin, X. Wei, H. Li, X. Zhao, A. Jiang and J. Yuan, Food Funct., 2018, Accepted Manuscript , DOI: 10.1039/C7FO01720K

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