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Bioactive Peptide Isolated from Casein Phosphopeptides Promotes Calcium Uptake in vitro and in vivo


Casein phosphopeptides (CPPs) have been demonstrated as a calcium chelate. Unfortunately, few studies had been reported on the effects of CPPs on Ca2+ uptake absorption mechanism and the bone metabolism. In this study, the monomer peptide RP-HPLC-isolated fraction (F6-1) possessing high calcium transport capacity in Caco-2 cell monolayers was separated and characterized. The effect of F6-1 on the Ca2+ absorption mechanisms in Caco-2 monolayers model and the bone metabolism in rats were investigated. The F6-1 was isolated by preparative and analytic RP-HPLC. Calcium transport results suggested that the rate of F6-1 Ca2+ transportation were approximately 2.57-, 2.87- and 2.38-fold higher than that of the control at 30, 60 and 120 min, respectively. The ultraviolet (UV) result indicated that intensity of UV absorption was changed because of the binding of Ca2+ to F6-1. transepithelial electric resistance (TEER) analysis and the expression of TRPV6 in Caco-2 cells showed that F6-1 was likely to influence transcellular pathway of intestinal Ca2+ absorption rather than paracellular pathway. Furthermore, F6-1 group (1% Ca, 0.03% F6-1) increased serum Ca2+ level, femur length and femur Ca, and decreased the serum alkaline phosphatase (ALP) levels and urinary pyridinoline content in Sprague-Dawley rat animal model, implying that F6-1 was beneficial to bone calcification. Overall, our results suggested that F6-1 had enhanced the Ca2+ transport in Caco-2 cells via affecting the transcellular pathway by up-regulating TRPV6 expression, F6-1 also had improved bone formation and prevent bone resorption to benefit bone health in rats, which provided a basis for using F6-1 for calcium supplements or functional foods.

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Publication details

The article was received on 31 Oct 2017, accepted on 03 Mar 2018 and first published on 05 Mar 2018

Article type: Paper
DOI: 10.1039/C7FO01709J
Citation: Food Funct., 2018, Accepted Manuscript
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    Bioactive Peptide Isolated from Casein Phosphopeptides Promotes Calcium Uptake in vitro and in vivo

    G. Liu, S. Sun, B. Guo, B. Miao, Z. Luo, Z. Xia, D. Ying, F. Liu, B. Guo, J. Tang, Y. Cao and J. Miao, Food Funct., 2018, Accepted Manuscript , DOI: 10.1039/C7FO01709J

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