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Urinary metabolomic study of antagonistic effect of Panax ginseng in rat with estrogen decline using ultra performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry

Abstract

Estrogens are biologically active steroid hormones mainly from the ovary by ovarian secretion of estrogen into the blood circulation to regulate or function at the distal target. Estrogens play an important role in the central nervous system, cardiovascular system and immune system, especially for postmenopausal women. Panax ginseng Mayer has been reported to relieve women menopausal symptoms and affect estrogen activities. However, its mechanism of estrogen regulation has not yet been clearly investigated. In this work, the ovariectomized rats were administered with P. ginseng decoction intragastrically for 8 weeks. Urine samples were analyzed by ultra performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS) to identify metabolites. The estrous cycle, body weight, uterine weight index and serum hormone are measured respectively. The results showed that P. ginseng significantly prolonged the estrous stage, decreased the body weight and serum luteinizing hormone (LH) and increased uterine weight index and serum estradiol (E2) of ovariectomized rats. A total of twelve potential biomarkers which levels changed markedly upon treatment had been identified based on metabolomics. A systematic networks of their corresponding pathways analysis indicated that the antagonistic effect of P. ginseng on ovariectomized rats mainly through regulating the steroid hormone metabolism, fatty acid biosynthesis, citric acid cycle and tryptophan metabolism. In conclusion, the study validated the antagonistic effect of P. ginseng in rat with estrogen decline and explored the metabolic and biochemical mechanisms involved.

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Publication details

The article was received on 25 Oct 2017, accepted on 31 Jan 2018 and first published on 02 Feb 2018


Article type: Paper
DOI: 10.1039/C7FO01680H
Citation: Food Funct., 2018, Accepted Manuscript
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    Urinary metabolomic study of antagonistic effect of Panax ginseng in rat with estrogen decline using ultra performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry

    H. Lin, Z. Liu, Z. Pi, L. Men, W. Chen and Z. Liu, Food Funct., 2018, Accepted Manuscript , DOI: 10.1039/C7FO01680H

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