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The effect of fucoidan on intestinal flora and intestinal barrier function in rats bearing breast cancer

Abstract

Recent research have showed that the intestinal flora are related to the occurrence and progress of breast cancer. This study was to investigate the effect of fucoidan on intestinal flora and intestinal barrier function in rats with 7, 12-Dimethyl Benz[a]anthracene(DMBA)-induced breast cancers. Sixty female Sprague-Dawley rats were randomly assigned to control group, model group, and the F1 and F2 groups, which were fed fucoidan at concentrations of 200 and 400 mg/kg•bw (body weight), respectively. Intestinal histopathological analysis was performed and 16S rDNA high-throughput sequencing was used to provide an overview of the intestinal flora composition. The contents of D-lactic acid (D-LA), diamine oxidase (DAO) and endotoxin in plasma were detected by ELISA. Expression levels of the tight junction (TJ) proteins, phosphorylated p38 MAPK and ERK1/2 were measured using western blotting. Our results suggested the intestinal wall of model group were damaged. However, after fucoidan intervention, the villi were gradually restored. ELISA showed the levels of plasma endotoxin, D-LA and DAO decreased in F1 and F2 groups compared to those in model group. Fucoidan treatment also increased the expressions of ZO-1, Occludin, Claudin-1 and Claudin-8. Furthermore, the expression levels of phosphorylated p38 MAPK and ERK1/2 were upregulated in fucoidan treatment groups. The results of 16S rDNA high-throughput sequencing indicated that fucoidan increased diversity of intestinal microbiota and induced changes in microbial composition.with increased Bacteroidetes/Firmicutes phylum ratio. In conclusion, the supplement of fucoidan could improve the fecal microbiota composition and repair the intestinal barrier function. The study suggested the use of fucoidan as an intestinal flora modulator for potential prevention of breast cancer.

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Publication details

The article was received on 25 Oct 2017, accepted on 09 Jan 2018 and first published on 10 Jan 2018


Article type: Paper
DOI: 10.1039/C7FO01677H
Citation: Food Funct., 2018, Accepted Manuscript
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    The effect of fucoidan on intestinal flora and intestinal barrier function in rats bearing breast cancer

    M. Xue, X. Ji, H. Liang, Y. Liu, B. Wang, L. Sun and W. Li, Food Funct., 2018, Accepted Manuscript , DOI: 10.1039/C7FO01677H

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