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Milk-derived bioactive peptides protect against oxidative stress in a Caco-2 cell model


Milk and milk-derived products are a relevant source of bioactive peptides which are also potential components of functional food. Bioactive peptides exert multiple actions including an antioxidant role. In the present paper, four synthetic peptides, (NPYVPR, AVPYPQR, KVLPVPEK, ARHPHPHLSFM) corresponding to milk-derived peptides, were studied. Although with different features, as revealed by RP-HPLC chromatography and MS analysis, the obtained peptides were shown to be taken up by Caco-2 cells arranged in an epithelial monolayer formation. The four peptides were all able to preserve cell viability against induced oxidative stress indicating that they might have a role on the control of oxidative stress. Therefore, estimation of total thiols and glutathione content were performed after cell treatment with oxidants like hydrogen peroxide (H2O2) or tert-butyl hydroperoxide (TbOOH). Peptides were able to prevent the decrease of both total thiols and glutathione induced by H2O2 or TbOOH and, in addition, they showed a protective effect on the thiol-related antioxidant enzymes thioredoxin reductase and glutathione reductase. Finally, they determined a decrease of ROS production induced by TbOOH in Caco-2 cells. The reported results highlight the relevant antioxidant role played by bioactive peptides in cells, which adds to other previously known properties.

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Publication details

The article was received on 20 Oct 2017, accepted on 09 Jan 2018 and first published on 10 Jan 2018

Article type: Paper
DOI: 10.1039/C7FO01646H
Citation: Food Funct., 2018, Accepted Manuscript
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    Milk-derived bioactive peptides protect against oxidative stress in a Caco-2 cell model

    F. Tonolo, M. Sandre, S. Ferro, A. Folda, V. Scalcon, G. Scutari, E. Feller, O. Marin, A. Bindoli and M. P. Rigobello, Food Funct., 2018, Accepted Manuscript , DOI: 10.1039/C7FO01646H

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