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Extraction of triterpenoids and phenolic compounds from Ganoderma lucidum: optimization study using the response surface methodology

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Abstract

The extraction of triterpenoids and phenolic compounds from Ganoderma lucidum was optimized by using the response surface methodology (RSM), using heat and ultrasound assisted extraction techniques (HAE and UAE). The obtained results were compared with that of the standard Soxhlet procedure. RSM was applied using a circumscribed central composite design with three variables (time, ethanol content, and temperature or ultrasonic power) and five levels. The conditions that maximize the responses (extraction yield, triterpenoids and total phenolics) were: 78.9 min, 90.0 °C and 62.5% ethanol and 40 min, 100.0 W and 89.5% ethanol for HAE and UAE, respectively. The latter was the most effective, resulting in an extraction yield of 4.9 ± 0.6% comprising a content of 435.6 ± 21.1 mg g−1 of triterpenes and 106.6 ± 16.2 mg g−1 of total phenolics. The optimized extracts were fully characterized in terms of individual phenolic compounds and triterpenoids by HPLC-DAD-ESI/MS. The recovery of the above-mentioned bioactive compounds was markedly enhanced using the UAE technique.

Graphical abstract: Extraction of triterpenoids and phenolic compounds from Ganoderma lucidum: optimization study using the response surface methodology

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Publication details

The article was received on 13 Oct 2017, accepted on 22 Nov 2017 and first published on 23 Nov 2017


Article type: Paper
DOI: 10.1039/C7FO01601H
Citation: Food Funct., 2018, Advance Article
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    Extraction of triterpenoids and phenolic compounds from Ganoderma lucidum: optimization study using the response surface methodology

    T. Oludemi, L. Barros, M. A. Prieto, S. A. Heleno, M. F. Barreiro and I. C. F. R. Ferreira, Food Funct., 2018, Advance Article , DOI: 10.1039/C7FO01601H

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