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Effect of bovine lactoferrin on L-DOPA absorption and metabolism in mice

Abstract

Bioactive natural products, habitually ingested with milk, or its derivative nutrients have been studied for their bioavailability. In this study, we investigated the effects of the co-administration of bovine milk-derived lactoferrin (bLF) and bioactive products, with a focus on catechol-O-methyltransferase (COMT), an enzyme in catechol metabolism. bLF showed inhibitory activity on COMT in vitro, and acidic pretreatment of bLF enhanced its inhibitory activity. Moreover, partial digested products of bLF by pepsin retained inhibitory activity. Based on these results, bLF was co-administered with levodopa (L-DOPA), which is a catechol compound and a precursor of dopamine, and the effect of bLF on the L-DOPA absorption and metabolism was investigated in a mouse model. The co-administration of L-DOPA and bLF alone showed no effect on the concentration of L-DOPA in plasma. However, with the additional administration of carbidopa, the concentration of L-DOPA was significantly enhanced. Further, the ratio of 3-O-methyldopa/L-DOPA significantly decreased. On the other hand, casein, which is a major milk protein, was not effective. In addition, COMT activity in the intestines was lowered with bLF administration. We concluded that the co-administration of bLF and carbidopa enhances the concentration of L-DOPA.

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Publication details

The article was received on 29 Sep 2017, accepted on 28 Mar 2018 and first published on 06 Apr 2018


Article type: Paper
DOI: 10.1039/C7FO01518F
Citation: Food Funct., 2018, Accepted Manuscript
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    Effect of bovine lactoferrin on L-DOPA absorption and metabolism in mice

    M. Ikeda, H. Iijima, I. Shinoda, H. Iwamoto and Y. Takeda, Food Funct., 2018, Accepted Manuscript , DOI: 10.1039/C7FO01518F

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