Jump to main content
Jump to site search


The critical role of light in moderating microbial stress due to mixtures of engineered nanomaterials

Author affiliations

Abstract

Exposure to light is a key parameter that influences the chemical interactions and microbial stress of engineered nanomaterials (ENMs). Yet, the photochemistry and phototoxic stress responses of many ENMs and ENM mixtures have not been adequately detailed. We exposed E. coli bacteria to binary combinations of n-TiO2 (a stable metal oxide) with different plasmonic metal ENMs (n-Ag, n-Au, or n-Pt) in a natural aqueous medium under light and dark conditions. Using ATP level and cell membrane integrity probes, we measure the toxic stress due to these ENM mixtures. We previously found that under dark conditions, n-TiO2 attenuates the toxic stress due to low concentrations of n-Ag (<20 μg L−1) via adsorption of Ag+. However, mixtures containing n-Au or n-Pt produce no difference in toxic stress responses with and without n-TiO2 in the dark. In contrast, under simulated solar irradiation, we observed that n-Ag, n-Au, and n-Pt each with n-TiO2 cause synergistic toxic stress. The n-Ag/n-TiO2 combination causes the greatest phototoxicity, followed by n-Au/n-TiO2 and then n-Pt/n-TiO2. Measurements of photocatalytic production of reactive oxygen species reveal that irradiation of n-Ag or n-Au with n-TiO2 yields synergistic production of superoxide anion and hydrogen peroxide. The photochemical interactions of these ENMs, governed by metal ENM solubility and localized surface plasmon resonance, provide mechanistic insight into the amplified photoactivity of mixtures.

Graphical abstract: The critical role of light in moderating microbial stress due to mixtures of engineered nanomaterials

Back to tab navigation

Supplementary files

Publication details

The article was received on 12 Jun 2017, accepted on 10 Nov 2017 and first published on 10 Nov 2017


Article type: Paper
DOI: 10.1039/C7EN00527J
Citation: Environ. Sci.: Nano, 2018, Advance Article
  •   Request permissions

    The critical role of light in moderating microbial stress due to mixtures of engineered nanomaterials

    C. M. Wilke, J. Gaillard and K. A. Gray, Environ. Sci.: Nano, 2018, Advance Article , DOI: 10.1039/C7EN00527J

Search articles by author

Spotlight

Advertisements