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Issue 15, 2018
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Relationship between copper(II) complexes with FomA adhesin fragments of F. nucleatum and colorectal cancer. Coordination pattern and ability to promote ROS production

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Abstract

The copper(II) ion binding of the Ac-KGHGNG-NH2 and Ac-PTVHNE-NH2 fragments of FomA adhesin from Fusobacterium nucleatum was studied using potentiometry, UV-Vis, CD, EPR and DFT techniques. The coordination pattern was described in a wide range of pH values. Ligands begin interactions with metal ions using imidazole nitrogen. At pH 6.8 (a value typical of the large intestine environment), the metal ion was coordinated by the 3N donor atoms {Nim, 2 × Namide} in both cases. However, the copper(II) ion was bound more effectively by the Ac-PTVHNE-NH2 peptide. The formation of reactive oxygen species (ROS) was studied by UV-Vis and fluorescence spectroscopy, as well as gel electrophoresis in the presence of H2O2 and/or ascorbic acid. The complexes generated ROS in the highest amounts among all compounds. Moreover, they stimulated the CT26 cell line (mouse colon carcinoma) to produce ROS which lead to oxidative stress. It was also determined that such radicals took part in the plasmid degradation mechanism.

Graphical abstract: Relationship between copper(ii) complexes with FomA adhesin fragments of F. nucleatum and colorectal cancer. Coordination pattern and ability to promote ROS production

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Publication details

The article was received on 31 Oct 2017, accepted on 08 Mar 2018 and first published on 13 Mar 2018


Article type: Paper
DOI: 10.1039/C7DT04103A
Citation: Dalton Trans., 2018,47, 5445-5458
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    Relationship between copper(II) complexes with FomA adhesin fragments of F. nucleatum and colorectal cancer. Coordination pattern and ability to promote ROS production

    M. K. Lesiów, U. K. Komarnicka, K. Stokowa-Sołtys, K. Rolka, A. Łęgowska, N. Ptaszyńska, R. Wieczorek, A. Kyzioł and M. Jeżowska-Bojczuk, Dalton Trans., 2018, 47, 5445
    DOI: 10.1039/C7DT04103A

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