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An artificial metalloenzyme for carbene transfer based on a biotinylated dirhodium anchored within streptavidin

Abstract

We report on artificial metalloenzymes that incorporate a biotinylated dirhodium core embedded within engineered streptavidin (Sav hereafter) variants. The resulting biohybrid catalyzes the carbene insertion in C–H bonds and olefins. Chemical- and genetic optimization allows to modulate the catalytic activity of the artificial metalloenzymes that are shown to be active in the periplasm of E. coli (up to 20 turnovers).

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Publication details

The article was accepted on 13 Apr 2018 and first published on 16 Apr 2018


Article type: Communication
DOI: 10.1039/C8CY00646F
Citation: Catal. Sci. Technol., 2018, Accepted Manuscript
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    An artificial metalloenzyme for carbene transfer based on a biotinylated dirhodium anchored within streptavidin

    J. Zhao, D. G. Bachmann, M. Lenz, D. Gillingham and T. R. Ward, Catal. Sci. Technol., 2018, Accepted Manuscript , DOI: 10.1039/C8CY00646F

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