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Issue 9, 2018
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An artificial metalloenzyme for carbene transfer based on a biotinylated dirhodium anchored within streptavidin

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Abstract

We report on artificial metalloenzymes that incorporate a biotinylated dirhodium core embedded within engineered streptavidin (Sav hereafter) variants. The resulting biohybrid catalyzes the carbene insertion in C–H bonds and olefins. Chemical- and genetic optimization allows to modulate the catalytic activity of the artificial metalloenzymes that are shown to be active in the periplasm of E. coli (up to 20 turnovers).

Graphical abstract: An artificial metalloenzyme for carbene transfer based on a biotinylated dirhodium anchored within streptavidin

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Publication details

The article was received on 30 Mar 2018, accepted on 13 Apr 2018 and first published on 16 Apr 2018


Article type: Communication
DOI: 10.1039/C8CY00646F
Citation: Catal. Sci. Technol., 2018,8, 2294-2298
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    An artificial metalloenzyme for carbene transfer based on a biotinylated dirhodium anchored within streptavidin

    J. Zhao, D. G. Bachmann, M. Lenz, D. G. Gillingham and T. R. Ward, Catal. Sci. Technol., 2018, 8, 2294
    DOI: 10.1039/C8CY00646F

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