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Influence of the nature of the support in the catalytic performance of CALB: experimental and theoretical evidences

Abstract

The immobilization of the lipase B of Candida antarctica (CALB) was carried out on various hydrophilic and hydrophobic supports through adsorption and covalent bond interactions. The catalytic performance of the biocatalysts was evaluated in the enantioselective esterification the R/S-ibuprofen with ethanol without co-solvents added. The present investigation demonstrates that the biocatalysts based on CALB immobilized by adsorption on hydrophobic supports (acrylic resin, polymethylmethacrylate, polystyrene, epoxy resin and polypropylene) possess higher conversion of ibuprofen and enantiomeric excess towards the S-enantiomer than CALB immobilized onto hydrophilic materials. Additionally, the enzyme loading is a key factor influencing the conversion of the substrate regardless the nature of the support material. High enzyme loading is detrimental for the specific activity of the biocatalysts. Experimental and molecular modeling investigations provided evidences of the influence of the water activity in the stereospecificity of the lipase.

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Publication details

The article was received on 03 Dec 2017, accepted on 04 Jun 2018 and first published on 07 Jun 2018


Article type: Paper
DOI: 10.1039/C7CY02466E
Citation: Catal. Sci. Technol., 2018, Accepted Manuscript
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    Influence of the nature of the support in the catalytic performance of CALB: experimental and theoretical evidences

    C. Jose, M. V. Toledo, P. Nicolas, V. L. Lassalle, M. L. Ferreira and L. E. Briand, Catal. Sci. Technol., 2018, Accepted Manuscript , DOI: 10.1039/C7CY02466E

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