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Issue 25, 2018
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Lysine-based amino-functionalized lipids for gene transfection: 3D phase behaviour and transfection performance

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Abstract

Based on previous work, the influence of the chain composition on the physical–chemical properties of five new transfection lipids (TH10, TT10, OH10, OT10 and OO10) containing the same lysine-based head group has been investigated in aqueous dispersions. For this purpose, the chain composition has been gradually varied from saturated tetradecyl (T, C14:0) and hexadecyl (H, C16:0) chains to longer but unsaturated oleyl (O, C18:1) chains with double bonds in the cis configuration. In this work, the lipid dispersions have been investigated in the absence and presence of the helper lipid DOPE and calf thymus DNA by small-angle and wide-angle X-ray scattering (SAXS/WAXS) supplemented by differential scanning calorimetry (DSC), attenuated total reflection Fourier-transform infrared spectroscopy (ATR-FTIR) and Fourier-transform Raman spectroscopy (FTRS). Lamellar and inverted hexagonal mesophases have been observed in single-component systems. In the binary mixtures, the aggregation behaviour changes with an increasing amount of DOPE from lamellar to cubic. The lipid mixtures with DNA show a panoply of mesophases. Interestingly, TT10 and OT10 form cubic lipoplexes, whereas OO10 complexes the DNA sandwich-like between lipid bilayers in a lamellar lipoplex. Surprisingly, the latter is the most effective lipoplex.

Graphical abstract: Lysine-based amino-functionalized lipids for gene transfection: 3D phase behaviour and transfection performance

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Publication details

The article was received on 24 Mar 2018, accepted on 17 May 2018 and first published on 21 May 2018


Article type: Paper
DOI: 10.1039/C8CP01922C
Citation: Phys. Chem. Chem. Phys., 2018,20, 17393-17405
  • Open access: Creative Commons BY license
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    Lysine-based amino-functionalized lipids for gene transfection: 3D phase behaviour and transfection performance

    S. Tassler, D. Pawlowska, C. Janich, J. Giselbrecht, S. Drescher, A. Langner, C. Wölk and G. Brezesinski, Phys. Chem. Chem. Phys., 2018, 20, 17393
    DOI: 10.1039/C8CP01922C

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