Jump to main content
Jump to site search


Spectroscopic evidence for hydration and dehydration of lipid bilayers upon interaction with metal ions: a new physical insight

Abstract

In this manuscript, we investigate the interactions of different metal ions with zwitterionic phospholipid bilayers of different chain lengths using a well-known membrane probe PRODAN by steady state and time resolved fluorescence spectroscopy. We used three zwitterionic lipids which are widely different in their phase transition temperature, namely, dipalmitoylphosphatidylcholine (DPPC), 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC), and 2-Oleoyl-1-palmitoyl-sn-glycero-3-phosphocholine (POPC) and salts of Zinc (Zn), Calcium (Ca) and Magnesium (Mg). The steady state and time resolved studies reveal that the affinity of metal ions follow the order Zn+2 > Ca+2 > Mg+2. The study further reveals that lipid membrane with unsaturated chain exhibits very small affinity towards metal ion. We find that metal ions Zn+2 and Ca+2 induce significant gelation in lipid bilayer possibly by dehydrating the lipid bilayer surface. The study also demonstrates that unlike Zn+2 and Ca+2, dehydration does not take place for Mg+2. The extreme hydration induced by Mg+2 is rationalized by tight hydration of Mg+2 and very high free energy barrier of Mg+2 to bind with lipid oxygen as compared to that of water molecules.

Back to tab navigation

Supplementary files

Publication details

The article was received on 19 Mar 2018, accepted on 16 Apr 2018 and first published on 16 Apr 2018


Article type: Paper
DOI: 10.1039/C8CP01774C
Citation: Phys. Chem. Chem. Phys., 2018, Accepted Manuscript
  •   Request permissions

    Spectroscopic evidence for hydration and dehydration of lipid bilayers upon interaction with metal ions: a new physical insight

    S. De, N. Kanwa, M. Ahmed and A. Chakraborty, Phys. Chem. Chem. Phys., 2018, Accepted Manuscript , DOI: 10.1039/C8CP01774C

Search articles by author

Spotlight

Advertisements