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Electron Transfer Rate Analysis of a Site-Specifically Wired Copper Oxidase

Abstract

Low electron transfer efficiency between redox enzymes and electrodes is one of the major bottlenecks in the improvement of enzyme based biofuel cells. Direct transfer of electrons between an enzyme redox center and an electrode is the preferred and most efficient method to prevent both coulombic and ohmic losses that decrease the power output of the cell. Site-specific wiring of redox enzymes using non-canonical amino acids incorporated into the enzymes can be used to control the enzyme’s orientation and distance from the electrode surface, thus providing optimal conditions for direct electron transfer. In this work we site-specifically wired the blue copper oxidase, CueO, onto a glassy carbon electrode, allowing direct electron transfer to either one of the enzyme’s redox centers. By measuring electron transfer rates to each of the redox centers, we demonstrated the importance of the anchoring site and length of the ‘wire’ molecule used for immobilization.

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Publication details

The article was received on 03 Jan 2018, accepted on 13 Feb 2018 and first published on 13 Feb 2018


Article type: Paper
DOI: 10.1039/C8CP00041G
Citation: Phys. Chem. Chem. Phys., 2018, Accepted Manuscript
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    Electron Transfer Rate Analysis of a Site-Specifically Wired Copper Oxidase

    O. Schlesinger, M. Pasi, R. Dandela, M. M. Meijler and L. Alfonta, Phys. Chem. Chem. Phys., 2018, Accepted Manuscript , DOI: 10.1039/C8CP00041G

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