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Issue 13, 2018
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An antibody-free assay for simultaneous capture and detection of glycoproteins by surface enhanced Raman spectroscopy

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Abstract

A sensitive, and single step reaction assay of glycoproteins in antibody-free mode was proposed on a 4-mercapto-phenylboronic acid (4-MPBA) modified silver nanoparticle film (AgNF) by using surface enhanced Raman scattering (SERS). 4-MPBA/AgNF was designed both as a glycoprotein-specific recognition biosensor and as a Raman reporter for the SERS platform. Variation in the ratio I1574/I1586 for 4-MPBA/AgNF in response to glycoprotein capture constitutes the SERS assay which exhibits high sensitivity and selectivity against nonglycoproteins and is shown to function in complex samples.

Graphical abstract: An antibody-free assay for simultaneous capture and detection of glycoproteins by surface enhanced Raman spectroscopy

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Publication details

The article was received on 19 Dec 2017, accepted on 23 Feb 2018 and first published on 26 Feb 2018


Article type: Paper
DOI: 10.1039/C7CP08478A
Citation: Phys. Chem. Chem. Phys., 2018,20, 8881-8886
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    An antibody-free assay for simultaneous capture and detection of glycoproteins by surface enhanced Raman spectroscopy

    D. Xie, W. F. Zhu, H. Cheng, Z. Y. Yao, M. Li and Y. L. Zhao, Phys. Chem. Chem. Phys., 2018, 20, 8881
    DOI: 10.1039/C7CP08478A

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