Issue 8, 2018

In situ examination of a charged amino acid-induced structural change in lipid bilayers by sum frequency generation vibrational spectroscopy

Abstract

The interactions between amino acids (AAs) and membranes represent various short-range and long-range interactions for biological phenomena; however, they are still poorly understood. In this study, we used cationic lysine and arginine as AA models, and systematically investigated the interactions between charged AAs and lipid bilayers using sum frequency generation vibrational spectroscopy (SFG-VS) in situ and in real time. The AA-induced dynamic structural changes of the lipid bilayer were experimentally monitored using the spectral features of CD2, CD3, the lipid head phosphate, and carbonyl groups in real time. Time-dependent SFG changes in the structure of the lipid bilayer provide direct evidence for the different interactions of lysine and arginine with the membrane. It was found that the discrepancy between lysine and arginine in binding with the lipid bilayer is due to the nature of the terminal functional groups. Arginine exhibits a more drastic impact on the membrane than lysine. SFG responses of the acyl chains, phosphate groups, and carbonyl groups provide evidence that the interaction between AAs and the membrane most likely follows an electrostatics and hydrogen bond-induced defect model. This work presents an exemplary method for comprehensive investigations of interactions between membranes and other functionally significant substances.

Graphical abstract: In situ examination of a charged amino acid-induced structural change in lipid bilayers by sum frequency generation vibrational spectroscopy

Supplementary files

Article information

Article type
Paper
Submitted
01 Nov 2017
Accepted
23 Jan 2018
First published
24 Jan 2018

Phys. Chem. Chem. Phys., 2018,20, 5657-5665

In situ examination of a charged amino acid-induced structural change in lipid bilayers by sum frequency generation vibrational spectroscopy

J. Zhang, W. Yang, J. Tan and S. Ye, Phys. Chem. Chem. Phys., 2018, 20, 5657 DOI: 10.1039/C7CP07389E

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