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Rapid localized crystallization of lysozyme by laser trapping

Abstract

Confining protein crystallization into a millimetre size was attained within 0.5 hr after stopping intense trapping laser irradiation of 1 hr, which shows excellent performance in spatial and temporal controllability compared to spontaneous nucleation. A continuous-wave near-infrared laser beam is tightly focused into a glass/solution inter-facial layer of supersaturated buffer solution of hen egg-white lysozyme (HEWL). The crystallization is not observed during laser trapping, while initiated by stopping the laser irradiation. The generated crystals are localized densely in a circle area with the diametre of a few millimetres around the focal spot and show specific directions of the optical axis of HEWL crystal. To interpret the unique crystallization, we propose a mechanism that nucleation and the following growth take place in a highly concentrated domain consisting of HEWL liquid-like clusters after turning off laser trapping.

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Publication details

The article was received on 13 Oct 2017, accepted on 03 Jan 2018 and first published on 03 Jan 2018


Article type: Communication
DOI: 10.1039/C7CP06990A
Citation: Phys. Chem. Chem. Phys., 2018, Accepted Manuscript
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    Rapid localized crystallization of lysozyme by laser trapping

    K. Yuyama, K. Chang, J. Tu, H. Masuhara and T. Sugiyama, Phys. Chem. Chem. Phys., 2018, Accepted Manuscript , DOI: 10.1039/C7CP06990A

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