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Issue 12, 2018
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Competitive DNA binding of Ru(bpy)2dppz2+ enantiomers studied with isothermal titration calorimetry (ITC) using a direct and general binding isotherm algorithm

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Abstract

While isothermal titration calorimetry (ITC) is widely used and sometimes referred to as the “gold standard” for quantitative measurements of biomolecular interactions, its usage has so far been limited to the analysis of the binding to isolated, non-cooperative binding sites. Studies on more complicated systems, where the binding sites interact, causing either cooperativity or anti-cooperativity between neighboring bound ligands, are rare, probably due to the complexity of the methods currently available. Here we have developed a simple algorithm not limited by the complexity of a binding system, meaning that it can be implemented by anyone, from analyzing systems of simple, isolated binding sites to complicated interactive multiple-site systems. We demonstrate here that even complicated competitive binding calorimetric isotherms can be properly analyzed, provided that ligand–ligand interactions are taken into account. As a practical example, the competitive binding interactions between the two enantiomers of Ru(bpy)2dppz2+ (Ru-bpy) and poly(dAdT)2 (AT-DNA) are analyzed using our new algorithm, which provided an excellent global fit for the ITC experimental data.

Graphical abstract: Competitive DNA binding of Ru(bpy)2dppz2+ enantiomers studied with isothermal titration calorimetry (ITC) using a direct and general binding isotherm algorithm

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Publication details

The article was received on 13 May 2017, accepted on 12 Dec 2017 and first published on 13 Dec 2017


Article type: Paper
DOI: 10.1039/C7CP03184J
Citation: Phys. Chem. Chem. Phys., 2018,20, 7920-7930
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    Competitive DNA binding of Ru(bpy)2dppz2+ enantiomers studied with isothermal titration calorimetry (ITC) using a direct and general binding isotherm algorithm

    A. K. F. Mårtensson and P. Lincoln, Phys. Chem. Chem. Phys., 2018, 20, 7920
    DOI: 10.1039/C7CP03184J

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