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Issue 14, 2018
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Application of protein crystallization methodologies to enhance the solubility, stability and monodispersity of proteins

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Abstract

Protein crystallization screens are commonly used to find specific crystallization conditions. In practice, undesirable clear drops are often ignored. We hypothesize that clear drops may be potentially useful for finding the conditions leading to proteins with high solubility and monodispersity . Based on this idea, we first tested three model proteins and confirmed that improved monodispersity could be observed in the clear drops. Next, we used this strategy to achieve the maximum solubility and monodispersity of a new protein (RNA binding motif 3, RBM3). We suggest that this strategy is useful not only for high-throughput screening of optimal solubilization conditions but also for screening of biopharmaceutical formulations.

Graphical abstract: Application of protein crystallization methodologies to enhance the solubility, stability and monodispersity of proteins

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Publication details

The article was received on 19 Dec 2017, accepted on 24 Feb 2018 and first published on 24 Feb 2018


Article type: Communication
DOI: 10.1039/C7CE02189E
Citation: CrystEngComm, 2018,20, 1923-1927
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    Application of protein crystallization methodologies to enhance the solubility, stability and monodispersity of proteins

    R. Zhou, X. Lu, C. Dong, F. Ahmad, C. Zhang and D. Yin, CrystEngComm, 2018, 20, 1923
    DOI: 10.1039/C7CE02189E

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