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Py A-cluster system for the detection and imaging of miRNAs in living cells through double-three-way junction formation

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Abstract

Herein, we describe an extended version of a fluorescence probe for detecting miRNAs through the novel application of a PyA-cluster system. By testing various (CG)n sequences in the middle of the oligonucleotide strand of the probe, we obtained an optimal sequence that formed a double-three-way-junction structure, with two PyA units positioned close together, in the presence of the target miRNA. This system readily detected the locations of target miRNAs in living cells and allowed visualization of structural changes through variations in the color of the fluorescence.

Graphical abstract: PyA-cluster system for the detection and imaging of miRNAs in living cells through double-three-way junction formation

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Publication details

The article was received on 18 May 2018, accepted on 10 Jun 2018 and first published on 12 Jun 2018


Article type: Communication
DOI: 10.1039/C8CC03982H
Citation: Chem. Commun., 2018, Advance Article
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    Py A-cluster system for the detection and imaging of miRNAs in living cells through double-three-way junction formation

    J. J. Ro, H. J. Lee and B. H. Kim, Chem. Commun., 2018, Advance Article , DOI: 10.1039/C8CC03982H

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