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Development of small molecule biosensors by coupling the recognition of the bacterial allosteric transcription factor with isothermal strand displacement amplification

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Abstract

Here, we demonstrate an easy-to-implement and general biosensing strategy by coupling the small-molecule recognition of the bacterial allosteric transcription factor (aTF) with isothermal strand displacement amplification (SDA) in vitro. Based on this strategy, we developed two biosensors for the detection of an antiseptic, p-hydroxybenzoic acid, and a disease marker, uric acid, using bacterial aTF HosA and HucR, respectively, highlighting the great potential of this strategy for the development of small-molecule biosensors.

Graphical abstract: Development of small molecule biosensors by coupling the recognition of the bacterial allosteric transcription factor with isothermal strand displacement amplification

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Publication details

The article was received on 04 Mar 2018, accepted on 08 Mar 2018 and first published on 10 Mar 2018


Article type: Communication
DOI: 10.1039/C8CC01764F
Citation: Chem. Commun., 2018, Advance Article
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    Development of small molecule biosensors by coupling the recognition of the bacterial allosteric transcription factor with isothermal strand displacement amplification

    Y. Yao, S. Li, J. Cao, W. Liu, K. Fan, W. Xiang, K. Yang, D. Kong and W. Wang, Chem. Commun., 2018, Advance Article , DOI: 10.1039/C8CC01764F

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