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Anti-Stokes fluorescence microscopy using direct and indirect dark state formation

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Abstract

Measurements on biological samples are often hampered by auto-fluorescence from inherent compounds in tissue or cells, limiting the achievable contrast. Both the signal of interest and the auto-fluorescence are usually detected on the Stokes side of the excitation laser. In this communication, we present two new microscopy modalities, based on the emission of a red-emitting DNA-stabilized silver nanocluster (DNA-AgNC). Its bright fluorescence can be generated on the anti-Stokes side of the readout laser, allowing easy spectral separation of the signal of interest from the Stokes side auto-fluorescence.

Graphical abstract: Anti-Stokes fluorescence microscopy using direct and indirect dark state formation

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Publication details

The article was received on 23 Feb 2018, accepted on 05 Apr 2018 and first published on 17 Apr 2018


Article type: Communication
DOI: 10.1039/C8CC01521J
Citation: Chem. Commun., 2018, Advance Article
  • Open access: Creative Commons BY license
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    Anti-Stokes fluorescence microscopy using direct and indirect dark state formation

    S. Krause, M. R. Carro-Temboury, C. Cerretani and T. Vosch, Chem. Commun., 2018, Advance Article , DOI: 10.1039/C8CC01521J

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