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Issue 36, 2018
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Gene assembly via one-pot chemical ligation of DNA promoted by DNA nanostructures

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Abstract

Current gene synthesis methods are driven by enzymatic reactions. Here we report the one-pot synthesis of a chemically-ligated gene from 14 oligonucleotides. The chemical ligation benefits from the highly efficient click chemistry approach templated by DNA nanostructures, and produces modified DNA that is compatible with polymerase enzymes.

Graphical abstract: Gene assembly via one-pot chemical ligation of DNA promoted by DNA nanostructures

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Publication details

The article was received on 28 Jan 2018, accepted on 20 Mar 2018 and first published on 17 Apr 2018


Article type: Communication
DOI: 10.1039/C8CC00738A
Citation: Chem. Commun., 2018,54, 4529-4532
  • Open access: Creative Commons BY license
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    Gene assembly via one-pot chemical ligation of DNA promoted by DNA nanostructures

    I. Manuguerra, S. Croce, A. H. El-Sagheer, A. Krissanaprasit, T. Brown, K. V. Gothelf and A. Manetto, Chem. Commun., 2018, 54, 4529
    DOI: 10.1039/C8CC00738A

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