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Issue 19, 2018
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Sensitive detection of alkaline phosphatase by dephosphorylation-initiated transcription reaction-mediated dual signal amplification

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Abstract

We develop a new fluorescence method for the sensitive detection of alkaline phosphatase (ALP) based on dephosphorylation-initiated transcription reaction-mediated dual signal amplification. This research demonstrates for the first time a significant improvement in ALP assay performance by using a nucleic acid amplification strategy, which enables sensitive detection of ALP activity in vitro and in cell extracts. Importantly, this method can be further applied for kinetic analysis and ALP inhibition assay.

Graphical abstract: Sensitive detection of alkaline phosphatase by dephosphorylation-initiated transcription reaction-mediated dual signal amplification

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Publication details

The article was received on 22 Jan 2018, accepted on 12 Feb 2018 and first published on 12 Feb 2018


Article type: Communication
DOI: 10.1039/C8CC00540K
Citation: Chem. Commun., 2018,54, 2413-2416
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    Sensitive detection of alkaline phosphatase by dephosphorylation-initiated transcription reaction-mediated dual signal amplification

    F. Ma, W. Liu, L. Liang, B. Tang and C. Zhang, Chem. Commun., 2018, 54, 2413
    DOI: 10.1039/C8CC00540K

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