Issue 25, 2018

2-Oxoglutarate regulates binding of hydroxylated hypoxia-inducible factor to prolyl hydroxylase domain 2

Abstract

Prolyl hydroxylation of hypoxia inducible factor (HIF)-α, as catalysed by the Fe(II)/2-oxoglutarate (2OG)-dependent prolyl hydroxylase domain (PHD) enzymes, has a hypoxia sensing role in animals. We report that binding of prolyl-hydroxylated HIF-α to PHD2 is ∼50 fold hindered by prior 2OG binding; thus, when 2OG is limiting, HIF-α degradation might be inhibited by PHD binding.

Graphical abstract: 2-Oxoglutarate regulates binding of hydroxylated hypoxia-inducible factor to prolyl hydroxylase domain 2

Supplementary files

Article information

Article type
Communication
Submitted
16 Jan 2018
Accepted
31 Jan 2018
First published
09 Mar 2018
This article is Open Access
Creative Commons BY license

Chem. Commun., 2018,54, 3130-3133

2-Oxoglutarate regulates binding of hydroxylated hypoxia-inducible factor to prolyl hydroxylase domain 2

M. I. Abboud, T. E. McAllister, I. K. H. Leung, R. Chowdhury, C. Jorgensen, C. Domene, J. Mecinović, K. Lippl, R. L. Hancock, R. J. Hopkinson, A. Kawamura, T. D. W. Claridge and C. J. Schofield, Chem. Commun., 2018, 54, 3130 DOI: 10.1039/C8CC00387D

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