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Issue 24, 2018
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Genetic incorporation of L-dihydroxyphenylalanine (DOPA) biosynthesized by a tyrosine phenol-lyase

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Abstract

L-Dihydroxyphenylalanine (DOPA) was biosynthesized by a tyrosine-phenol lyase from catechol, pyruvate, and ammonia in Escherichia coli, and the biosynthesized amino acid was directly incorporated into proteins. Three biochemical experiments with mutant proteins containing DOPA confirmed the genetic incorporation of biosynthesized DOPA, and revealed its potential for various biochemical applications.

Graphical abstract: Genetic incorporation of l-dihydroxyphenylalanine (DOPA) biosynthesized by a tyrosine phenol-lyase

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Publication details

The article was received on 12 Jan 2018, accepted on 12 Feb 2018 and first published on 26 Feb 2018


Article type: Communication
DOI: 10.1039/C8CC00281A
Citation: Chem. Commun., 2018,54, 3002-3005
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    Genetic incorporation of L-dihydroxyphenylalanine (DOPA) biosynthesized by a tyrosine phenol-lyase

    S. Kim, B. H. Sung, S. C. Kim and H. S. Lee, Chem. Commun., 2018, 54, 3002
    DOI: 10.1039/C8CC00281A

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