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Issue 31, 2018
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A genetically encoded 19F NMR probe for lysine acetylation

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Abstract

Advances in acetylated protein–protein/DNA interactions depend on the development of a novel NMR (nuclear magnetic resonance) probe to study the conformational changes of acetylated proteins. However, the method for detecting the acetylated protein conformation is underdeveloped. Herein, an acetyllysine mimic has been exploited for detecting the conformational changes of acetylated p53–protein/DNA interactions by genetic code expansion and 19F NMR. This 19F NMR probe shows high structural similarity to acetyllysine and could not be deacetylated by sirtuin deacetylase in vitro/vivo. Moreover, acetylation of p53 K164 is reported to be deacetylated by SIRT2 for the first time.

Graphical abstract: A genetically encoded 19F NMR probe for lysine acetylation

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Publication details

The article was received on 23 Dec 2017, accepted on 08 Mar 2018 and first published on 09 Mar 2018


Article type: Communication
DOI: 10.1039/C7CC09825A
Citation: Chem. Commun., 2018,54, 3879-3882
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    A genetically encoded 19F NMR probe for lysine acetylation

    F. Zhang, Q. Zhou, G. Yang, L. An, F. Li and J. Wang, Chem. Commun., 2018, 54, 3879
    DOI: 10.1039/C7CC09825A

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