Jump to main content
Jump to site search


Synthetic cells produce a quorum sensing chemical signal perceived by Pseudomonas aeruginosa

Author affiliations

Abstract

Recent developments in bottom-up synthetic biology (e.g., lipid vesicle technology integrated with cell-free protein expression systems) allow the generation of semi-synthetic minimal cells (in short, synthetic cells, SCs) endowed with some distinctive capacities of natural cells. In particular, such approaches provide technological tools and conceptual frameworks for the design and engineering of programmable SCs capable of communicating with natural cells by exchanging chemical signals. Here we describe the generation of giant vesicle-based SCs which, via gene expression, synthesize in their aqueous lumen an enzyme that in turn produces a chemical signal. The latter is a small molecule, which is passively released in the medium and then perceived by the bacterium Pseudomonas aeruginosa, demonstrating that SCs and bacteria can communicate chemically. The results pave the way to a novel basic and applied research area where synthetic cells can communicate with natural cells, for example for exploring minimal cognition, developing chemical information technologies, and producing smart and programmable drug-producing/drug-delivery systems.

Graphical abstract: Synthetic cells produce a quorum sensing chemical signal perceived by Pseudomonas aeruginosa

Back to tab navigation

Supplementary files

Publication details

The article was received on 18 Dec 2017, accepted on 04 Jan 2018 and first published on 04 Jan 2018


Article type: Communication
DOI: 10.1039/C7CC09678J
Citation: Chem. Commun., 2018, Advance Article
  •   Request permissions

    Synthetic cells produce a quorum sensing chemical signal perceived by Pseudomonas aeruginosa

    G. Rampioni, F. D’Angelo, M. Messina, A. Zennaro, Y. Kuruma, D. Tofani, L. Leoni and P. Stano, Chem. Commun., 2018, Advance Article , DOI: 10.1039/C7CC09678J

Search articles by author

Spotlight

Advertisements