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Issue 17, 2018
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Isothermal multiple displacement amplification of DNA templates in minimally buffered conditions using phi29 polymerase

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Abstract

The isothermal amplification of DNA in minimally buffered conditions allows to perform and monitor nucleic acid amplification with minimal technological and operative requirements. We show in this work how phi29 can operate multiple displacement amplification in minimally buffered conditions producing, as a readout, pH shifts attaining subunits of pH.

Graphical abstract: Isothermal multiple displacement amplification of DNA templates in minimally buffered conditions using phi29 polymerase

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Publication details

The article was received on 19 Dec 2017, accepted on 02 Feb 2018 and first published on 02 Feb 2018


Article type: Communication
DOI: 10.1039/C7CC09609G
Citation: Chem. Commun., 2018,54, 2158-2161
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    Isothermal multiple displacement amplification of DNA templates in minimally buffered conditions using phi29 polymerase

    E. Tenaglia, Y. Imaizumi, Y. Miyahara and C. Guiducci, Chem. Commun., 2018, 54, 2158
    DOI: 10.1039/C7CC09609G

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