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A gold nanoparticle-based four-color proximity immunoassay for one-step, multiplexed detection of protein biomarkers using ribonuclease H signal amplification

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Abstract

A novel gold nanoparticle-based four-color fluorescence proximity immunoassay is developed for one-step, multiplexed detection of protein biomarkers using ribonuclease H signal amplification. This assay could detect proteins with detection limits down to pg mL−1 and a 5-log dynamic range. Furthermore, it is suitable for rapid, direct quantification of multiple proteins in biological samples.

Graphical abstract: A gold nanoparticle-based four-color proximity immunoassay for one-step, multiplexed detection of protein biomarkers using ribonuclease H signal amplification

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Publication details

The article was received on 07 Dec 2017, accepted on 22 Jan 2018 and first published on 23 Jan 2018


Article type: Communication
DOI: 10.1039/C7CC09404C
Citation: Chem. Commun., 2018, Advance Article
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    A gold nanoparticle-based four-color proximity immunoassay for one-step, multiplexed detection of protein biomarkers using ribonuclease H signal amplification

    J. Xu, M. Shi, W. Chen, Y. Huang, L. Fang, L. Yao, S. Zhao, Z. Chen and H. Liang, Chem. Commun., 2018, Advance Article , DOI: 10.1039/C7CC09404C

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