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Issue 37, 2018
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Covalent and selective immobilization of GST fusion proteins with fluorophosphonate-based probes

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Abstract

Using GST fusion protein tags is an attractive approach for protein immobilization. Here we report that pyrimidine-based small-molecule probes with a fluorophosphonate reactive group could specifically react with the tyrosine-111 residue of the Schistosoma japonicum GST (sjGST) tag, and these probes could rapidly and site-selectively immobilize sjGST fusion proteins while preserving their activities.

Graphical abstract: Covalent and selective immobilization of GST fusion proteins with fluorophosphonate-based probes

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Publication details

The article was received on 18 Nov 2017, accepted on 09 Feb 2018 and first published on 15 Mar 2018


Article type: Communication
DOI: 10.1039/C7CC08888D
Citation: Chem. Commun., 2018,54, 4661-4664
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    Covalent and selective immobilization of GST fusion proteins with fluorophosphonate-based probes

    X. Wang, T. Guo, J. Chen, X. Li, Y. Zhou and Z. Pan, Chem. Commun., 2018, 54, 4661
    DOI: 10.1039/C7CC08888D

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