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SpyCatcher–SpyTag mediated in situ labelling of progeny baculovirus with quantum dots for tracking viral infection in living cells

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Abstract

A non-invasive labelling strategy is proposed to label baculovirus via genetic insertion of a SpyTag into the viral glycoprotein, followed by specific conjugation with the SpyCatcher protein on modified quantum dots (QDs) through an isopeptide bond. The labelling method is convenient and efficient and shows little attenuation of viral infectivity. Therefore, it is a biologically compatible technique for tracking viral infection.

Graphical abstract: SpyCatcher–SpyTag mediated in situ labelling of progeny baculovirus with quantum dots for tracking viral infection in living cells

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Publication details

The article was received on 18 Nov 2017, accepted on 05 Jan 2018 and first published on 08 Jan 2018


Article type: Communication
DOI: 10.1039/C7CC08880A
Citation: Chem. Commun., 2018, Advance Article
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    SpyCatcher–SpyTag mediated in situ labelling of progeny baculovirus with quantum dots for tracking viral infection in living cells

    X. Ke, Y. Zhang, F. Zheng, Y. Liu, Z. Zheng, Y. Xu and H. Wang, Chem. Commun., 2018, Advance Article , DOI: 10.1039/C7CC08880A

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