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Issue 23, 2018
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SypHer3s: a genetically encoded fluorescent ratiometric probe with enhanced brightness and an improved dynamic range

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Abstract

We designed a genetically encoded ratiometric fluorescent probe, SypHer3s, with enhanced brightness and optimized pKa, which responds to pH changes in different cellular compartments. SypHer3s was successfully utilized for imaging the pH dynamics in mitochondria of living neurons and in quantitative pH measurement in zebrafish embryos.

Graphical abstract: SypHer3s: a genetically encoded fluorescent ratiometric probe with enhanced brightness and an improved dynamic range

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Publication details

The article was received on 15 Nov 2017, accepted on 20 Feb 2018 and first published on 20 Feb 2018


Article type: Communication
DOI: 10.1039/C7CC08740C
Citation: Chem. Commun., 2018,54, 2898-2901
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    SypHer3s: a genetically encoded fluorescent ratiometric probe with enhanced brightness and an improved dynamic range

    Y. G. Ermakova, V. V. Pak, Y. A. Bogdanova, A. A. Kotlobay, I. V. Yampolsky, A. G. Shokhina, A. S. Panova, R. A. Marygin, D. B. Staroverov, D. S. Bilan, H. Sies and V. V. Belousov, Chem. Commun., 2018, 54, 2898
    DOI: 10.1039/C7CC08740C

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